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biolog phenotype microarray (pm) experiment  (Biolog Inc)

 
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    Biolog Inc biolog phenotype microarray (pm) experiment
    Biolog Phenotype Microarray (Pm) Experiment, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+experiments/phenotype+microarrays/pmc11626823-221-30-31
    Average 90 stars, based on 1 article reviews
    biolog phenotype microarray (pm) experiment - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: On the function of TRAP substrate-binding proteins: the isethionate-specific binding protein IseP
    Article Snippet: DSF experiments were conducted using a QuantStudio 3 Real-Time PCR System (Applied Biosystems/Thermo Fisher Scientific). .. For experiments using the Phenotype MicroArray PM4A Biolog screen, the compounds were dissolved in 50 ml of 50 mM Tris pH 8.0, 150 mM NaCl buffer, before 7.5 ml was used in each replicate (Biolog does not disclose the exact concentrations in their screens). .. Each well was loaded with 25 ml of conditions comprising 15 mM OaIseP in 50 mM Tris pH 8.0, 150 mM NaCl together with 10X SYPRO Orange dye (Invitrogen) and either 0.1 or 5 mM isethionate.

    Article Title: Pseudomonas aeruginosa Increases the Sensitivity of Biofilm-Grown Staphylococcus aureus to Membrane-Targeting Antiseptics and Antibiotics
    Article Snippet: .. In the experiments described above using the Biolog Phenotype MicroArray panels, the compounds tested were added at the same time as the microbes were inoculated into the medium; thus, there was limited time for the bacteria to form a biofilm before exposure to the candidate agents. .. Therefore, we next tested whether P. aeruginosa supernatant could increase the efficacy of the compounds we identified in the Biolog screen against preformed early (6-h) S. aureus biofilms.

    Article Title: Klebsiella oxytoca inhibits Salmonella infection through multiple microbiota-context-dependent mechanisms.
    Article Snippet: .. The Biolog experiments were performed according to the manufacturer’s descriptions with the phenotypic microarray plates PM1 and PM2a, which are 96-well plates coated with lyophilized sugars in the usual range microbes require for growing (exact concentrations are not provided by the manufacturer). .. One day before the assay was performed, bacteria of interest were streaked on an R2A agar plate (Difco) to deplete the remaining nutrient stores from the cells.

    Bacteria:

    Article Title: Pseudomonas aeruginosa Increases the Sensitivity of Biofilm-Grown Staphylococcus aureus to Membrane-Targeting Antiseptics and Antibiotics
    Article Snippet: .. In the experiments described above using the Biolog Phenotype MicroArray panels, the compounds tested were added at the same time as the microbes were inoculated into the medium; thus, there was limited time for the bacteria to form a biofilm before exposure to the candidate agents. .. Therefore, we next tested whether P. aeruginosa supernatant could increase the efficacy of the compounds we identified in the Biolog screen against preformed early (6-h) S. aureus biofilms.

    other:

    Article Title: Membrane properties modulation by SanA: implications for xenobiotic resistance in Salmonella Typhimurium
    Article Snippet: The susceptibility of mutant and the parental strain to 240 chemical compounds was determined in three independent experiments using the Phenotype MicroArray (PM) PM11-PM20 (Biolog), as described in a previous study ( ).

    Article Title: On the function of TRAP substrate-binding proteins: the isethionate-specific binding protein IseP
    Article Snippet: Samples were exchanged into 200 mM ammonium acetate using Bio-Rad Micro Bio-Spin columns (final sample concentration of 20 μM), before infusion using gold-coated nanospray capillaries, prepared in-house, with an approximate orifice size of 1 μm.

    Article Title: P162: Changes of café-au-lait spots intensity by selumetinib in neurofibromatosis type I*
    Article Snippet: Methods: The experiments employed the Biolog Phenotype Mammalian Microarray (PM-M) technology to assess the metabolic response of lymphoblastoid cells from individualswith PMS to the candidate compounds (IGF-1 and hGH).We analyzedpreliminary PM-Mdata from a cohort of 48 patientswith PMS and 50 controls using the non-parametric Mann–Whitney two-sided test to identify the highest and lowest responders to IGF-1 and hGH.

    Article Title: Resolving the Combinatorial Complexity of Smad Protein Complex Formation and Its Link to Gene Expression.
    Article Snippet: Experiments were performed in biolog (D) Microarray-based gene expression analysis of Hepa1-6 cells treated (red) or divided into 12 groups by k-means clustering.

    Article Title: A systematic evaluation of Mycobacterium tuberculosis Genome-Scale Metabolic Networks
    Article Snippet: The Biolog Phenotype MicroArray experiments classification used were obtained from Lofthouse and colleagues, 2013.



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    Image Search Results


    A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

    Journal: Journal of Alzheimer's Disease Reports

    Article Title: Transcriptomic Analysis of Alzheimer’s Disease Pathways in a Pakistani Population 1

    doi: 10.3233/ADR-230146

    Figure Lengend Snippet: A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

    Article Snippet: The oligonucleotide microarray experiments were conducted by EpigenDx (Boston, MA) using the Affymetrix U133 Plus 2.0 Array platform, which has comprehensive coverage of the whole transcribed human genome on a single array.

    Techniques: Derivative Assay, Microarray, Expressing, Activation Assay